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Literature summary extracted from

  • Deeg, M.A.; Davitz, M.A.
    Glycosylphosphatidylinositol-phospholipase D: a tool for glycosylphosphatidylinositol structural analysis (1995), Methods Enzymol., 250, 630-640.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.50 Nonidet P-40 stimulation Homo sapiens
3.1.4.50 Nonidet P-40 stimulation Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.50 overview Homo sapiens
3.1.4.50 overview Bos taurus

General Stability

EC Number General Stability Organism
3.1.4.50 stable over a range of 0.01-0.1% detergent, ionic detergents such as SDS are inhibitory, and bicarbonate buffer is inhibitory Homo sapiens
3.1.4.50 stable over a range of 0.01-0.1% detergent, ionic detergents such as SDS are inhibitory, and bicarbonate buffer is inhibitory Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.50 1,10-phenanthroline
-
 Bos taurus
3.1.4.50 1,10-phenanthroline
-
 Homo sapiens
3.1.4.50 Bicarbonate buffer
-
 Bos taurus
3.1.4.50 Bicarbonate buffer
-
 Homo sapiens
3.1.4.50 EGTA
-
 Bos taurus
3.1.4.50 EGTA
-
 Homo sapiens
3.1.4.50 lipid A
-
 Bos taurus
3.1.4.50 lipid A
-
 Homo sapiens
3.1.4.50 lysophosphatidic acid
-
 Bos taurus
3.1.4.50 lysophosphatidic acid
-
 Homo sapiens
3.1.4.50 phosphatidic acid
-
 Bos taurus
3.1.4.50 phosphatidic acid
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.50 Ca2+ activation Homo sapiens
3.1.4.50 Ca2+ activation Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.50 100000
-
 gel filtration Bos taurus
3.1.4.50 110000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.50 Bos taurus
-
-
-
3.1.4.50 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.50
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.50 plasma
-
 Homo sapiens
-
3.1.4.50 plasma
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.50 additional information no substrate: phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol derived from HNO2-cleaved mfVSG, overview on substrate specificity Homo sapiens ?
-
 ?
3.1.4.50 additional information no substrate: phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol derived from HNO2-cleaved mfVSG, overview on substrate specificity Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.50 monomer 1 * 110000 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.50 7.4
-
 assay at Homo sapiens
3.1.4.50 7.4
-
 assay at Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.50 5 8
-
 Homo sapiens
3.1.4.50 5 8
-
 Bos taurus